Journal: iScience

Article Title: Dietary fiber reduces mortality from secondary sepsis in a murine model of Clostridioides difficile infection

doi: 10.1016/j.isci.2026.115258

Figure Lengend Snippet: Plasma markers of systemic inflammation and kidney injury in CDI mice across diets (A) PCoA of Canberra distances of mean-normalized sepsis/immune marker concentrations. Points are colored by diet, and shapes indicate whether mice were humanely euthanized due to clinical sickness or survived until the experimental endpoint. Vectors show the correlation of each measured factor with PC1 and PC2, with the vector length indicating the relative strength of the correlation. Only statistically significant vectors are shown (multiple regression with Benjamini-Hochberg multiple test correction, p.adj. < 0.05). (B) Plasma concentration of each marker that significantly differed between diets (blood urea nitrogen (BUN), and immune factors sCD14, CXCL1, IL-10, IL-1B, IL-6, and TNF-a) at sacrifice. Pairwise comparisons of concentrations between diets were calculated using Kruskal-Wallis and Dunn’s post hoc tests, with p -value corrections conducted via Benjamini and Hochberg. Boxplot lines (from top to bottom) depict the 75 th , 50 th (median), and 25 th percentiles, with lines extending from the top/bottom of the boxplot indicating the largest/smallest observation within ±1.5∗IQR (inter-quartile range). P-value significance (∗∗∗∗: p < 0.0001, ∗∗∗: p < 0.001, ∗∗: p < 0.01, and ∗: p < 0.05).

Article Snippet: Soluble CD14 (sCD14) levels were quantified using the Quantikine Mouse sCD14 ELISA Kit (Catalog No. MC140, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Marker, Plasmid Preparation, Concentration Assay

Journal: Schizophrenia research

Article Title: Discordant patterns of bacterial translocation markers and implications for innate immune imbalances in schizophrenia

doi: 10.1016/j.schres.2013.05.018

Figure Lengend Snippet: Markers of bacterial translocation, sCD14 and LBP, were inter-correlated. A. sCD14 and LBP marker correlations in cohort 1 based in Baltimore, MD, U.S.A. are shown. Multiple linear regressions included age, gender, race, smoking status, and assay plate designation. B. sCD14 and LBP marker correlations in cohort 2 based in Cologne, Germany, are shown. Multiple linear regressions included age, gender, and assay plate designation. AP refers to antipsychotic. [For color reproduction]

Article Snippet: 2.2 Laboratory procedures sCD14 and LBP levels were measured according to the manufacturer’s protocol using commercially available kits (Human sCD14 Quantikine ELISA kit, R&D Systems, Minneapolis, MN, U.S.A.; Multispecies Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Canton, MA, U.S.A.).

Techniques: Translocation Assay, Marker

Journal: Schizophrenia research

Article Title: Discordant patterns of bacterial translocation markers and implications for innate immune imbalances in schizophrenia

doi: 10.1016/j.schres.2013.05.018

Figure Lengend Snippet: sCD14 and LBP levels in cohort 1 cases compared to controls. A. sCD14 levels were elevated in schizophrenia and bipolar disorder compared to controls. B. LBP levels were not different between cases and controls, but levels in schizophrenia were higher than levels in bipolar disorder. [For color reproduction]

Article Snippet: 2.2 Laboratory procedures sCD14 and LBP levels were measured according to the manufacturer’s protocol using commercially available kits (Human sCD14 Quantikine ELISA kit, R&D Systems, Minneapolis, MN, U.S.A.; Multispecies Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Canton, MA, U.S.A.).

Techniques:

Journal: Schizophrenia research

Article Title: Discordant patterns of bacterial translocation markers and implications for innate immune imbalances in schizophrenia

doi: 10.1016/j.schres.2013.05.018

Figure Lengend Snippet: sCD14 and LBP exhibited sex-specific patterns. A. sCD14 levels were elevated in female individuals with schizophrenia compared to males. B. LBP levels were elevated in females compared to males in both the control and schizophrenia groups. [For color reproduction]

Article Snippet: 2.2 Laboratory procedures sCD14 and LBP levels were measured according to the manufacturer’s protocol using commercially available kits (Human sCD14 Quantikine ELISA kit, R&D Systems, Minneapolis, MN, U.S.A.; Multispecies Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Canton, MA, U.S.A.).

Techniques: Control

Journal: Schizophrenia research

Article Title: Discordant patterns of bacterial translocation markers and implications for innate immune imbalances in schizophrenia

doi: 10.1016/j.schres.2013.05.018

Figure Lengend Snippet: Associations of sCD14 and LBP with gluten IgG, CRP, BMI scores and tTG IgG.

Article Snippet: 2.2 Laboratory procedures sCD14 and LBP levels were measured according to the manufacturer’s protocol using commercially available kits (Human sCD14 Quantikine ELISA kit, R&D Systems, Minneapolis, MN, U.S.A.; Multispecies Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Canton, MA, U.S.A.).

Techniques: Control

Journal: Schizophrenia research

Article Title: Discordant patterns of bacterial translocation markers and implications for innate immune imbalances in schizophrenia

doi: 10.1016/j.schres.2013.05.018

Figure Lengend Snippet: Overview of coordinated activation of sCD14 and LBP during bacterial translocation. Following episodes of increased GI permeability, enteric bacteria may be exposed to the immune cells of the lamina propria and to systemic circulation. LBP interacts directly with the LPS endotoxin of Gram-negative bacteria and transports LPS monomers to sCD14 and CD14 bound to macrophage membranes. sCD14 is manufactured by immune cells of the GI mucosa, whereas LBP is synthesized by GI epithelial cells. Both markers are also produced by the liver (Heumann and Roger, 2002; Kitchens and Thompson, 2005; Miyake, 2004; Sandler and Douek, 2012; Stehle et al., 2012). Dietary gluten may impact components of these pathways and based on our study results is included in a hypothetical role in this diagram. [For color reproduction]

Article Snippet: 2.2 Laboratory procedures sCD14 and LBP levels were measured according to the manufacturer’s protocol using commercially available kits (Human sCD14 Quantikine ELISA kit, R&D Systems, Minneapolis, MN, U.S.A.; Multispecies Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Canton, MA, U.S.A.).

Techniques: Activation Assay, Translocation Assay, Permeability, Bacteria, Synthesized, Produced

Journal: JCI insight

Article Title: A human-origin probiotic cocktail ameliorates aging-related leaky gut and inflammation via modulating the microbiota/taurine/tight junction axis.

doi: 10.1172/jci.insight.132055

Figure Lengend Snippet: Figure 3. Probiotics treatment reduces inflammation in peritoneal macrophages and intestine of older obese mice. (A–C) LPS-induced inflammatory response in terms of mRNA expression of IL-6 (A), TNF-α (B), and IL-1β (C) was reduced in primary macrophages isolated from peritoneal cavity of older HFD-fed mice treated with probiotics (n = 8) compared with their controls (n = 6). (D–H) In addition, the expression of proinflammatory markers such as IL-6 (D), TNF-α (E), and IL-1β (F) were decreased, while antiinflammatory genes like IL-10 (G) and TGF-β (H) mRNA expressions were increased in the colon of probiotic-fed older obese mice (n = 8) compared with their controls (n = 6). (I and J) In addition, systemic leaky gut markers such as LPS binding protein (LBP) (I) and soluble CD14 (J) were reduced in the serum of probiotic-fed older obese mice (n = 7) compared with their controls (n = 6). Values are mean of n = 6–8 in each group, and data are shown as mean ± SEM. *P < 0.05; **P < 0.01, ***P < 0.001 by Student t-test (A–J).

Article Snippet: To determine the impact of probiotics feeding on systemic leaky gut, markers such as LBP (catalog HK205-01, Hycult Biotech) and sCD14 (catalog MC140, R&D Systems) were measured in serum using ELISA kits and following manufacturer instructions.

Techniques: Probiotics, Expressing, Isolation, Binding Assay

Journal: Nutrients

Article Title: Relationship of Diet to Gut Microbiota and Inflammatory Biomarkers in People with HIV

doi: 10.3390/nu14061221

Figure Lengend Snippet: Inflammatory biomarkers (ng/mL) by dietary patterns.

Article Snippet: Tumour soluble necrosis factor sTNFR2 (DRT200, R & D Systems, Bio-Techne Corporation, Minneapolis, MN, USA), C-reactive protein (CRP) (DCRP00, Quantikine ELISA kit, R & D Systems, Minneapolis, MN, USA), sCD14 (AbClonal, Wuhan, China), sCD163 (AbClonal, Wuhan, China), FABP2/IFABP (Boster Biological Technology, Wuhan, China), D-dimers (Ray Biotech, Norcross, GA, USA), LTA (Abbexa, Cambridge, UK), LBP (Boster Biolo-gical Technology, Wuhan, China).

Techniques:

Journal: Nutrients

Article Title: Relationship of Diet to Gut Microbiota and Inflammatory Biomarkers in People with HIV

doi: 10.3390/nu14061221

Figure Lengend Snippet: Inflammatory biomarkers (ng/mL) by dietary patterns in MSM.

Article Snippet: Tumour soluble necrosis factor sTNFR2 (DRT200, R & D Systems, Bio-Techne Corporation, Minneapolis, MN, USA), C-reactive protein (CRP) (DCRP00, Quantikine ELISA kit, R & D Systems, Minneapolis, MN, USA), sCD14 (AbClonal, Wuhan, China), sCD163 (AbClonal, Wuhan, China), FABP2/IFABP (Boster Biological Technology, Wuhan, China), D-dimers (Ray Biotech, Norcross, GA, USA), LTA (Abbexa, Cambridge, UK), LBP (Boster Biolo-gical Technology, Wuhan, China).

Techniques: